instructions
Gradient Mixer GM-1
stirrer blade (6) lifts the liquid entering the mixing reservoir.
The motor unit is supplied for operation from 100–120 or
220–240 V AC, 50 or 60 Hz.
1. Introduction
The Gradient Mixer GM-1 (120 V, Code No. 19-0495-01,
230 V Code No. 19-0485-01) consists of two identical
reservoirs mounted on a base plate; connection between the
reservoirs is made via a valve. Mounted above the mixing
reservoir is a motor unit which drives a stirrer blade. The
outlet from the mixing reservoir to the column is fitted with
a flow control valve, FCV-1. The Gradient Mixer is
conveniently supported on a laboratory stand. Capillary
tubing, 2 m, is also supplied.
THE STIRRER BLADE (6) is secured to the motor axle by a
nylon screw (5). It is unnecessary to remove the screw to
release the stirrer blade.
THE CONTROL VALVE (12) regulates the flow of liquid
between the two reservoirs. It is fully open when screwed out
one complete revolution from the closed position.
THE FLOW CONTROL VALVE (18) is a needle valve
designed for shut-off as well as flow control. The valve
nipple accepts capillary tubing 1.6–1.9 mm o.d. Eluent flow
is regulated with the large knob (A). The line on this knob
and the markings on the valve housing can be used as a flow
rate guide. The valve can thus be opened to a previously
determined position to obtain a given flow rate. To obtain
reproducible flow rates the valve should always be returned
to the shut position before setting.
2. Safety
Warning!
When using hazardous chemicals, all suitable protective
measures such as protective glasses, must be taken.
3. Unpacking
Unpack the equipment carefully and check the contents of
the carton against the packing list.
THE STAND MOUNT (21) may be used to support the
Gradient Mixer on a laboratory stand. When not required,
it may be removed from the base plate (11) by unscrewing
the retaining knob (20).
4. Description
THE BASE PLATE (11)* is a precision machined
polypropylene block on which the reservoir cylinders (7) are
mounted.
5. Installation
Sealing between the base plate and the cylinders is provided
by O-rings (8).
Connection of tubing to the flow control valve
1. Cut the tubing cleanly at a 45° angle.
2. Remove the screw cap and slip it over the tubing.
THE RESERVOIR CYLINDERS (7) are straight
polymethylpentene tubes threaded at one end and with air
inlets at the other end. Each cylinder has a capacity of 300 ml
and is graduated to 250 ml at 25 ml intervals to give a rapid
check of the volume of liquid remaining in the reservoirs.
The mixing reservoir carries the motor unit (1, 2) and the
other reservoir is fitted with a snap-on lid (3).
3. Remove the rubber cone with the help of the metal
stopper.
4. Push the tubing through the rubber cone until it projects
about 1 cm.
THE MOTOR UNIT (1, 2) consists of a fully encapsulated
low speed 250 or 300 rpm synchronous motor mounted in
polypropylene housing. A phasing condensor ensures that
the direction of stirring is always the same and that the
5. Seat the tubing in the nipple. Slide the cone along the
tubing, into the nipple.
6. Finger tighten the screw cap.
* Numbers refer to the enclosed Spare Parts drawing.
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The figure indicates the correct locations of the different
parts of the Gradient Mixer. For replacements please order
according to the spare part list below using the appropriate
code number.
9. Spare parts
Caution!
Only spare parts approved or supplied by
GE Healthcare Bio-Sciences AB may be used
for maintaining and servicing of Gradient Mixer GM-1.
DetaiI No.
Designation
Code No.
Material
No. per pack
1
Motor unit (complete with
detail Nos. 3, 4, 5) 110 V
Motor unit (complete) 230 V
Lid
Switch
Screw
Stirrer blade
Reservoir cylinder
O-ring
18-1121-66
A*
1
2
3
4
5
6
7
8
18-1121-67
19-0452-01
A*
B
1
1
19-0455-01
19-0457-01
19-0364-01
A
D
E
1
1
2
11
12
13
14
15
Base plate
Control valve (complete)
Knob
Screw
O-ring
19-0462-01
A*
1
16
17
18
Foot
O-ring
19-7320-01
19-0680-01
19-0049-01
F
E
A*
4
10
1
FIow controI vaIve FCV-1
(complete)
19
20
Sealing plug
Retaining knob
19-0039-01
19-0489-01
E
G
5
1
21
22
23
Stand mount
Tubing
Stopper
19-0463-01
19-0040-01
19-0752-01
H
I
H
1
10 m
10
* material of main component.
Materials
A
Polypropylene
B Polyvinylchloride
C
Polyamide
D Polymethylpentene (TPX09)
E
F
Fluororubber
Polyurethane
G Superpolyoxymethylene
H Stainless steel
I
Polyethylene
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10. Important user information
Meaning:
Warranty and liability
Consult the instruction manual to avoid personal injury or
damage to the product or other equipment.
GE Healthcare Bio-Sciences AB guarantees that the product
delivered has been thoroughly tested to ensure that it meets
its published specifications. The warranty included in the
conditions of delivery is valid only if the products has been
installed and used according to the instructions supplied by
GE Healthcare Bio-Sciences AB.
Warning!
The Warning sign is used to call attention to the necessity to
follow an instruction in detail to avoid personal injury. Be
sure not to proceed until the instructions are clearly
understood and all stated conditions are met.
GE Healthcare Bio-Sciences AB shall in no event be liable for
incidental or consequential damages, including without
limitations, lost profits, loss of income, loss of business
opportunities, loss of use and other related exposures,
however caused, arising from the faulty and incorrect use of
the product.
Caution!
The Caution sign is used to call attention to instructions or
conditions that shall be followed to avoid damage to the
product or other equipment. Be sure not to proceed until the
instructions are clearly understood and all stated conditions
are met.
Note:
The Note is used to indicate information important for
trouble free or optimal use of the product. Should you have
any comments on this instruction, we will be pleased to
receive them at:
GE Healthcare Bio-Sciences AB
S-751 82 Uppsala, Sweden
to order:
Asia Pacific Tel: +852 2811 8693 Fax: +852 2811 5251 Australasia Tel: +61 2 9899 0999 Fax: +61 2 9899 7511 Austria Tel: 01 576 0616 20 Fax: 01 576 0616 27 Belgium Tel: 0800 73 888 Fax: 03 272 1637
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America Tel: +55 11 3933 7300 Fax: +55 11 3933 7315 Middle East and Africa Tel: +30 2 10 96 00 687 Fax: +30 2 10 96 00 693 Netherlands Tel: 0165 580 410 Fax: 0165 580 401 Norway Tel: 2318 5800 Fax: 2318
6800 Portugal Tel: 21 417 7035 Fax: 21 417 3184 Russia & other C.I.S. & N.I.S. Tel: +7 (095) 232 0250,956 1137 Fax: +7 (095) 230 6377 South East Asia Tel: 60 3 8024 2080 Fax: 60 3 8024 2090 Spain Tel: 93 594 49 50
Fax: 93 594 49 55 SwedenTel: +46 18 612 19 00 Fax: +46 18 612 19 10 Switzerland Tel: 01 802 81 50 Fax: 01 802 81 51 UK Tel: 0800 616 928 Fax: 0800 616 927 USA Tel: +1 800 526 3593 Fax: +1 877 295 8102
GE Healthcare are trademarks of General Electric Company. GE Healthcare Bio-Sciences AB Björkgatan 30, SE-751 84 Uppsala, Sweden. GE Healthcare Bio-Sciences Ltd GE Healthcare Place, Little Chalfont,
Buckinghamshire HP7 9NA, England. GE Healthcare Bio-Sciences Corp 800 Centennial Avenue, PO Box 1327, Piscataway, NJ 08855 USA. GE Healthcare Europe GmbH Munzinger Strasse 9, D-79111 Freiburg, Germany.
GE Healthcare Bio-Sciences KK Sanken Building, 3-25-1, Hyakunincho, Shinjuku-ku, Tokyo 169-0073, Japan. All goods and services are sold subject to the terms and conditions of sale of the company within
the General Electric Company group that supplies them. A copy of these terms and conditions is available on request.
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